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KMID : 0903620010420050527
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Journal of the Korean Society for Horticultural Science
2001 Volume.42 No. 5 p.527 ~ p.532
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Development and Application of SCAR Markers Related to Cytoplasmic Factor of Male Sterility in Onion (Allium cepa L.)
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양태진/Yang, Tae Jin
조광수/우종규/권영석/Cho, Kwang Soo/Woo, Jong Gyu/Kwon, Young Seok
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Abstract
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This study was carried out to develop sequence characterized amplified regions (SCARs) markers related to the cytoplasmic factor of male sterility and to apply them to onion breeding program using marker-assisted selection (MAS). Sequencing of the intergenic region between tRNA-Thr and tRNA-Leu in chloroplast DNA of onion revealed deletion of about 100 bp in S cytoplasm plants as compared with N cytoplasm. SCAR markers based on difference in the sequence of intergenic region, could be used to distinguish N and S cytoplasm of onion. Among them, MS1/MS2, MS1/MS4 and MS7/MS8 SCAR markers were reliable to identify the CMS factor. Percentage of the S cytoplasmic factor in populations of the F, hybrid cultivars and open-pollinated cultivar ´Daegwallyung No. 1´ were 100% and 26%, respectively. For application of MAS, the use of 0.5N NaOH in DNA extraction was reliable and simple as compared with the other methods. This method could be used to identify cyotoplasmic factor for 100 plants per day.
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